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- Title
Effects of nitric oxide scavengers on thermoinhibition of seed germination in Arabidopsis thaliana.
- Authors
Hossain, K. K.; Itoh, R. D.; Yoshimura, G.; Tokuda, G.; Oku, H.; Cohen, M. F.; Yamasaki, H.
- Abstract
Plant seeds sometimes do not germinate at elevated temperature. The thermoinhibition mechanisms of seed germination have yet not revealed. Here we describe a chemical approach to improve seed germination at high temperature. We compared the temperature response of germination between wild-type Arabidopsis thaliana and its T-DNA insertion mutant ΔAtGLB3 that lacks a functional gene encoding GLB3, a homologue of bacterial truncated Hb (trHb). Under optimal temperature conditions (e.g. 22°C), the seeds of ΔAtGLB3 and the wild type germinated at a frequency near 100%. In contrast, at 32°C the seeds of ΔAtGLB3 did not germinate while wild-type seeds retained the same high germination frequency. The germination of ΔAtGLB3 at 32°C was partially restored by supplementation with the nitric oxide-specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO; cPTIO), 3-(3,4-dihydroxycinnamoyl)quinic acid, bovine serum Hb, or isoprene. The results presented in this study suggest that chemical scavengers for reactive nitrogen species potentially improve seed germination at high temperature.
- Subjects
GERMINATION; NITRIC oxide; ARABIDOPSIS thaliana; HIGH temperatures; GENETIC code
- Publication
Russian Journal of Plant Physiology, 2010, Vol 57, Issue 2, p222
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443710020093