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- Title
High-throughput screening methods for nitrilases.
- Authors
Xue, Ya-Ping; Yang, Yue-Kai; Lv, Sheng-Zhi; Liu, Zhi-Qiang; Zheng, Yu-Guo
- Abstract
Nitrilases have been widely acknowledged as important alternatives to chemical catalysts, as they have been proved to transform an immense variety of nitriles under mild conditions and often in a stereoselective or regioselective manner. In the discovery of new nitrilases to establish viable industrial processes, screening plays an important role in identifying which subset of candidates contains a nitrilase of interest from a collection of organisms, clone banks, or enzyme libraries. However, the traditional methods for evaluating the nitrilases are a time-consuming, laborious, and costly process and have been regarded as a bottleneck in developing these nitrilases as industrial biocatalysts. In the past few years, a number of high-throughput screening methods have been developed for rapid evaluation and identification of nitrilases. Here, we review the various methodologies developed for high-throughput screening of nitrilases and focus on their advantages and limitations.
- Subjects
NITRILASES; STEREOSELECTIVE reactions; BIOCATALYSIS; HYDROLASES; METHODOLOGY
- Publication
Applied Microbiology & Biotechnology, 2016, Vol 100, Issue 8, p3421
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-016-7381-3