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- Title
基于 LC-MS 代谢组学解析凡纳滨对虾在微冻 贮藏过程中的代谢变化与品质的相关性.
- Authors
杨凯莹; 潘创; 郝淑贤; 陈胜军; 刘书成; 杨贤庆
- Abstract
To investigate the mechanism of quality deterioration of superchilled shrimp, metabolites change of Pacific white shrimp (Litopenaeus vannamei) during superchilling storage at -3 ℃ (0, 10, 20, 30 d) were investigated through liquid chromatography-mass spectrometry non targeted metabolomics. The key metabolites were screened by the correlation analysis between the potential biomarkers and the quality indices (including water holding capability, texture, pH, myofibrillar protein content, surface hydrophobicity and turbidity ). The results showed that significant differences were observed in the whole metabolic composition of shrimp muscle during superchilling storage. According to the OPLS-DA model, 79 significant different metabolites were screened from the shrimp muscle at different superchilling storage times. These different metabolites notably altered with the extension of time. The main metabolic pathways involved were biosynthesis of amino acids, biosynthesis of alkaloids derived from histidine and purine, lysine biosynthesis, citrate cycle (TCA cycle) and biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid. The quality indices showed that water holding capability, hardness, springiness and myofibrillar protein content gradually decreased during storage, while pH, surface hydrophobicity and turbidity gradually increased. The pathway analysis combined with correlation analysis revealed that L-lysine, L-aspartic acid, citric acid and isocitric acid were used as the key metabolites related to the metabolites for the quality changes of shrimp muscle during superchilling storage. This study revealed the metabolic characteristics of superchilled shrimp muscle at the molecular level, providing a theoretical basis for quickly and accurately reflecting the actual-time quality of shrimp muscle.
- Publication
Journal of Dalian Ocean University, 2023, Vol 38, Issue 5, p874
- ISSN
2095-1388
- Publication type
Article
- DOI
10.16535/j.cnki.dlhyxb.2023-014