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- Title
Production of 5,8-dihydroxy-9,12( Z, Z)-octadecadienoic acid from linoleic acid by whole recombinant Escherichia coli cells expressing diol synthase from Aspergillus nidulans.
- Authors
Seo, Min-Ju; Shin, Kyung-Chul; Oh, Deok-Kun
- Abstract
Diol synthase from Aspergillus nidulans was cloned and expressed in Escherichia coli. Recombinant E. coli cells expressing diol synthase from A. nidulans converted linoleic acid to a product that was identified as 5,8-dihydroxy-9,12( Z, Z)-octadecadienoic acid by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The recombinant cells and the purified enzyme showed the highest activity for linoleic acid among the fatty acids tested. The optimal reaction conditions for the production of 5,8-dihydroxy-9,12( Z, Z)-octadecadienoic acid from linoleic acid using whole recombinant E. coli cells expressing diol synthase were pH 7.5, 35°C, 250 rpm, 5 g l linoleic acid, 23 g l cells, and 20% (v/v) dimethyl sulfoxide in a 250-ml baffled flask. Under these optimized conditions, whole recombinant cells expressing diol synthase produced 4.98 g l 5,8-dihydroxy-9,12( Z, Z)-octadecadienoic acid for 150 min without detectable byproducts, with a conversion yield of 99% (w/w) and a productivity of 2.5 g l h. This is the first report on the biotechnological production of dihydroxy fatty acid using whole recombinant cells expressing diol synthase.
- Subjects
LINOLEIC acid; ESCHERICHIA coli; GENE expression in bacteria; SYNTHASES; GLYCOL synthesis; ASPERGILLUS nidulans; RECOMBINANT microorganisms
- Publication
Applied Microbiology & Biotechnology, 2014, Vol 98, Issue 17, p7447
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-014-5709-4