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- Title
Analysis of gut bacterial community composition in obese and lean Indian participants by denaturing gradient gel electrophoresis.
- Authors
Bahadur, Tej; Chaudhry, Rama; Bamola, Vishwa Deepak; Chutani, Alka Mohan; Verma, Anil Kumar; Paul, Jaishree
- Abstract
Coronavirus disease-2019 (COVID-19) is a global public health emergency and has affected more than 200 countries in the world. It was declared as a Public Health Emergency of International Concern by the end of January 2020 and a pandemic in March 2020. In addition to the public health challenges, this pandemic has created another parallel pandemic of mental health problems. Furthermore, the existing mental health problems have also been seen to exacerbate owing to this pandemic. The mental health problems are both seen among the community members and the health-care providers those who are in the continuous forefront of service provision in health-care settings. Ayurveda, the ancient medical doctrine of human civilization, delineates some of the tenets that can be understood and are pertinent to the current situation. Some of these tenets could be useful in mitigating the mental health issues that the current world is grappling with owing to COVID-19. Concepts such as Sadvrutta, Achara Rasayana, Adharma, and Prajnaaparadha hold contemporary relevance in the context of COVID-19. MATERIALS AND METHODS: Subjects with similar dietary habits were enrolled in the study. Fecal samples were collected from each individuals and DNA was extracted. Polymerase chain reaction (PCR) was performed to amplify the 16S ribosomal RNA gene (V1-V5 region) followed by GC clamp PCR (V3 region) for the same. PCR products were run on Denaturing Gradient Gel Electrophoresis (DGGE) system and DGGE sketches were analyzed in Gel Compar II version 6.6 software (Applied Maths, Belgium) to assess the gut microbial diversity. RESULTS AND CONCLUSION: The results showed variation in the gut microbial profiles among obese and lean individuals and revealed more microbial diversity in the lean as compared to obese individuals. These observations indicate that BMI is a contributing factor for the difference in gut bacterial profile of obese and lean subjects and that support the role of gut microbiota in obesity.
- Subjects
BACTERIAL communities; GEL electrophoresis; MENTAL health; POLYMERASE chain reaction; MICROBIAL diversity
- Publication
Indian Journal of Health Sciences & Biomedical Research KLEU, 2021, Vol 14, Issue 1, p46
- ISSN
2542-6214
- Publication type
Article
- DOI
10.4103/kleuhsj.kleuhsj_273_20