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- Title
IMMUNOLOGICAL BIOMARKERS FOR THE EARLY DIAGNOSIS OF IRAQI NEONATAL BACTERIAL SEPTICEMIA (EARLY AND LATE ONSET).
- Authors
Threaf, Mohammed Fluihh; Tektook, Nihad Khalawe; Pirko, Eptissam Younan
- Abstract
Neonatal septicemia (NNS) (both early and late onset sepsis) is an important common cause of mortality and morbidity among Iraqi neonates. In the first hours of NNS both cellular and humeral immune systems are activated important various molecules are released in neonate serum such as Procalcitonin; C-reactive protein and Interleukin as (IL-8). Therefore the current study is aimed to determine some important immunological Biomarkers (immunoglobulin’s as (IgG, IgM and IgA); Complement component (C3 and C4); Interleukin (IL8) and C-reactive protein), which are considered as a golden tools for the early diagnosis both types of neonatal Septicemia (early and late onset). Two ml of blood was collected from one hundred and sixty - six children (60 females and 106 males, aged 1-30 days) suspected of Neonatal Septicemia were included in these study and (50) healthy neonates, during the period from (January to July) 2018 in IBAN Al-Baladi Pediatric Hospital-Baghdad. All common bacteria isolated were identified by gram stain and Vitek system, Complement component (C3 and C4), immunoglobulin’s as (IgG, IgM and IgA) were measured by Singles Radial Immunodiffusion test, Interleukin-8 level was determined by ELISA as well as C-reactive protein by agglutination test. Out of the 166 suspected cases 106 (64%) were males and 60 (36%) were females, among the 56 positive blood culture cases 32(57%) were males more than 24 (43%) females. The results of the current study found that gram negative bacteria (84%) were isolated more than gram positive bacteria (14%). The (56) cases of NNS were divided into two age groups : early onset neonatal Septicemia (EONS) aged ≤7 days and late onset neonatal Septicemia (LONS) with aged >7days. Both gram negative and positive bacteria were isolated (75 and 58%) isolated from early onset neonatal Septicemia more than late onset neonatal Septicemia (25 and 42%), respectively. More bacterial gram positive isolated in both EONS and LONS was Staph.aureus 7(12.5%) and 4(7.14%) were more bacterial gram positive isolated, whilst Pseudomonas aeruginosa 11 (19.6%) and 4(7.14%) respectively were more bacterial gram negative isolated. Male cases in both groups of neonatal Septicemia (EOS and LOS) were more [15(47%) and 17( 53%)] respectively than females [11(46%) and 13 (54%)] respectively. Result showed that the means concentration of (IgA; IgG; IgM were 16.0 ± 5.3; 350.5 ± 76.7; 28.9 ± 12.0 mg/dl, respectively which were lower than the mean concentrations in control 20.3 ± 7.5; 512.5 ± 79.9 and 16.6 ± 7.20 mg/dl, respectively, and complement component level (C3 and C4) as (56.6 ± 14.5 and 13.6 ± 5.2) mg/dl compare to control (77.7 ± 12.5 and 17.7 ± 3.0) mg/dl also the level of interleukin-8 was (61.15±69.50) compare to control (7.50±1.53).
- Publication
Biochemical & Cellular Archives, 2018, Vol 18, Issue 2, p2547
- ISSN
0972-5075
- Publication type
Article