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- Title
Targeted ablation of TRAF6 inhibits skeletal muscle wasting in mice.
- Authors
Paul, Pradyut K.; Gupta, Sanjay K.; Bhatnagar, Shephali; Panguluri, Siva K.; Darnay, Bryant G.; Yongwon Choi; Kumar, Ashok
- Abstract
Skeletal muscle wasting is a major human morbidity, and contributes to mortality in a variety of clinical settings, including denervation and cancer cachexia. In this study, we demonstrate that the expression level and autoubiquitination of tumor necrosis factor (α) receptor adaptor protein 6 (TRAF6), a protein involved in receptor-mediated activation of several signaling pathways, is enhanced in skeletal muscle during atrophy. Skeletal muscle--restricted depletion of TRAF6 rescues myofibril degradation and preserves muscle fiber size and strength upon denervation. TRAF6 mediates the activation of JNK1/2, p38 mitogen--activated protein kinase, adenosine monophosphate-activated protein kinase, and nuclear factor κB, and induces the expression of muscle-specific E3 ubiquitin ligases and autophagy-related molecules in skeletal muscle upon denervation. Inhibition of TRAF6 also preserves the orderly pattern of intermyofibrillar and subsarcolemmal mitochondria in denervated muscle. Moreover, depletion of TRAF6 prevents cancer cachexia in an experimental mouse model. This study unveils a novel mechanism of skeletal muscle atrophy and suggests that TRAF6 is an important therapeutic target to prevent skeletal muscle wasting.
- Subjects
TUMOR necrosis factor receptors; MUSCLES; PROTEINS; PROTEIN kinases; ADENOSINES; NF-kappa B
- Publication
Journal of Cell Biology, 2010, Vol 191, Issue 7, p1395
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.201006098