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- Title
A Quantitative ELISA Protocol for Detection of Specific Human IgG against the SARS-CoV-2 Spike Protein.
- Authors
Vernet, Rémi; Charrier, Emily; Grogg, Julien; Mach, Nicolas
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide pandemic with at least 3.8 million deaths to date. For that reason, finding an efficient vaccine for this virus quickly became a global priority. The majority of vaccines now marketed are based on the SARS-CoV-2 spike protein that has been described as the keystone for optimal immunization. In order to monitor SARS-CoV-2 spike-specific humoral responses generated by immunization or infection, we have developed a robust and reproducible enzyme-linked immunosorbent assay (ELISA) protocol. This protocol describes a method for quantitative detection of IgG antibodies against the SARS-CoV-2 spike protein using antigen-coated microtiter plates. Results showed that antibodies could be quantified between the range of 1.953 ng/mL to 500 ng/mL with limited inter- and intra-assay variability.
- Subjects
SARS-CoV-2; IMMUNOGLOBULIN G; ENZYME-linked immunosorbent assay
- Publication
Vaccines, 2021, Vol 9, Issue 7, p770
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines9070770