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- Title
Galectin-3, a damage-associated molecular pattern, in tears of patients with vernal keratoconjunctivitis.
- Authors
Ito, Yousuke; Usui-Ouchi, Ayumi; Ebihara, Nobuyuki
- Abstract
Purpose: Galectin-3 is a damage-associated molecular pattern (DAMPs), released from damaged or dying cells. In this study, we investigated the concentration and source of galectin-3 in the tears of patients with vernal keratoconjunctivitis (VKC) and evaluated whether the concentration of galectin-3 in tears represents a biomarker of corneal epithelial damage. Study design: Clinical and experimental. Methods: We measured the concentration of galectin-3 in tear samples from 26 patients with VKC and 6 healthy controls by enzyme-linked immunosorbent assay (ELISA). The expression of galectin-3 in cultured human corneal epithelial cells (HCEs) stimulated with or without tryptase or chymase was investigated by polymerase chain reaction (PCR), ELISA, and Western blotting. We also estimated the concentration of galectin-3 in the supernatants of cultured HCEs induced to necrosis. Finally, we investigated whether recombinant galectin-3 induced the expression of various genes related to cell migration or the cell cycle in HCEs by using microarray analysis. Results: High concentrations of galectin-3 were detected in the tears of patients with VKC. The concentration showed significant correlation with the severity of corneal epithelial damage. Stimulation of cultured HCEs with various concentrations of tryptase or chymase had no effect on the expression of galectin-3. However, high concentrations of galectin-3 were detected in the supernatants of necrotic HCEs. Recombinant human galectin-3 induced various cell migration- and cell cycle-related genes. Conclusion: The concentrations of galectin-3 in the tears of patients with VKC may represent a biomarker of the severity of corneal epithelial damage.
- Subjects
ALLERGIC conjunctivitis; GALECTINS; ENZYME-linked immunosorbent assay; POLYMERASE chain reaction; CELL migration
- Publication
Japanese Journal of Ophthalmology, 2023, Vol 67, Issue 4, p431
- ISSN
0021-5155
- Publication type
Article
- DOI
10.1007/s10384-023-00994-9