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- Title
Proteomic Analysis of Human Lens Epithelial Cells Exposed to Microwaves.
- Authors
Hong-Wu Li; Ke Yao; Hong-Ying Jin; Li-Xia Sun; De-Qiang Lu; Yi-Bo Yu
- Abstract
Abstract Purpose To study proteomic changes in human lens epithelial cells (HLECs) exposed to 1800-MHz Global System for Mobile Communication (GSM)-like microwaves. Methods In three separate experiments, HLECs were exposed and sham-exposed (six dishes each) to 1800-MHz GSM-like radiation for 2 h. The specific absorption rates were 1.0, 2.0, or 3.5 W/kg. Immediately after radiation, the proteome was extracted from the HLECs. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis(2-DE; silver staining) and PDQuest 2-DE analysis software were used to separate and analyze the proteome of exposed and sham-exposed HLECs. Four differentially expressed protein spots were selected and identified by using electrospray ionization tandem mass spectrometry (ESI-MS-MS). Results When the protein profiles of exposed cells were compared with those of sham-exposed cells, four proteins were detected as upregulated. After analysis by ESI-MS-MS and through a database search, heat-shock protein (HSP) 70 and heterogeneous nuclear ribonucleoprotein K (hnRNP K) were determined to be upregulated in the exposed cells. Conclusions Two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry may be a powerful tool for screening potential electromagnetic-reaction protein markers. HSP70 and hnRNP K are involved in the stress reaction of HLECs exposed to microwaves. These cell responses are nonthermal effects of the electromagnetic field. Jpn J Ophthalmol 2007;51:412–416 © Japanese Ophthalmological Society 2007
- Subjects
EPITHELIAL cells; GSM communications; MICROWAVES; RADIATION; POLYACRYLAMIDE gel electrophoresis; HEAT shock proteins; GEL electrophoresis
- Publication
Japanese Journal of Ophthalmology, 2007, Vol 51, Issue 6, p412
- ISSN
0021-5155
- Publication type
Article
- DOI
10.1007/s10384-007-0483-9