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- Title
A single UVB exposure increases the expression of functional KIT in human melanocytes by up-regulating MITF expression through the phosphorylation of p38/CREB.
- Authors
Mizutani, Yuki; Hayashi, Nobukazu; Kawashima, Makoto; Imokawa, Genji
- Abstract
KIT is an essential receptor that modulates melanocyte function and whose function is disrupted in several pigmentary disorders. However, little is known about the effects of a single UVB exposure on the expression of KIT and two important regulatory transcription factors, MITF and AP-2α, in human melanocytes. We found that a single UVB exposure of human melanocytes induces an early decrease and a subsequent increase in functional KIT expression in concert with up-regulated MITF expression. The increased MITF expression was accompanied by a markedly stimulated and prolonged phosphorylation of p38/CREB. The UVB-stimulated expression of KIT could be completely abolished by a p38 inhibitor, concomitant with a reduced phosphorylation of CREB and a down-regulation of MITF expression. Interestingly, in non-UVB exposed human melanocytes, a MEK inhibitor stimulated the phosphorylation of p38/CREB which was associated with an increased production of MITF and KIT in a pattern similar to that induced by UVB. These findings indicate that UVB stimulates functional KIT expression in human melanocytes via the up-regulation of MITF which is, in turn, due to the activation of p38 and CREB.
- Subjects
PIGMENTATION disorders; MELANOCYTES; EPITHELIAL cells; PHOSPHORYLATION; TRANSCRIPTION factors
- Publication
Archives of Dermatological Research, 2010, Vol 302, Issue 4, p283
- ISSN
0340-3696
- Publication type
Article
- DOI
10.1007/s00403-009-1007-x