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- Title
Fabrication And Characterization of Carrageenan/Gelatin Hydrogel For Future Use in Airway Epithelium In Vitro Model.
- Authors
Karim, Nashihah Ab; Izan, Muhammad Firdaus Fairuz; Fauzi, Mh Busra; Mobarak, Nadhratun Naim; Lokanathan, Yogeswaran
- Abstract
Introduction: Respiratory diseases cause a major global health impact. The airway epithelium, which acts as a frontline defence, is one of the most common targets for inhaled allergens, irritants, or microorganisms to enter the respiratory system. In tissue engineering, hydrogel is commonly used as a scaffold due to its ability to sustain a 3D structure and allow mechanical support for the cells. The combination of carrageenan (carr) and gelatine (gel) hydrogel with suitable physicochemical and mechanical properties plays a part in supporting the in vitro model formation. Thus, developing an ideal biomaterial scaffold for in vitro testing system can improve the mucosalization capability of human airway epithelial cells. The objective of the study is to optimize and characterize the fabricated hybrid hydrogel formulation for ideal physicochemical and mechanical properties. Method: The hydrogel was fabricated from kappa carrageenan (carr) and bovine gelatin (gel). 2% and 5% gel solutions were mixed with 2% carr solution in this study. Different ratios of carr/gel-based hydrogel were used to fabricate the hydrogel, which is carr/gel (0:10), (2:8), (4:6) and (6:4). Then, the carr/gel-based hydrogels were cross-linked with genipin, and their physicochemical and mechanical properties such as biodegradation, porosity, water contact angle, gel thickness, swelling studies, viscosity, tensile strength, and biocompatibility will be characterized. Expected Result: The varied concentration of gelatin and ratio of the fabricated hybrid hydrogel may be caused by different physicochemical and mechanical results. Conclusion: The carr/gel-based hydrogel may be a promising biomaterial scaffold for future use in the airway epithelium in vitro model.
- Subjects
CARRAGEENANS; HYDROGELS; GELATIN; EPITHELIUM; AIRWAY (Anatomy)
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p19
- ISSN
1675-8544
- Publication type
Article