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- Title
Phytochemical Screening and Cytotoxicity of Crude Extracts from the Seed of M. oleifera against Breast, Liver and Prostate Cancer Cell lines.
- Authors
Karim, Nurul Ashikin Abd; Rukayadi, Yaya; Hamid, Hazrulizawati Abd; Razis, Ahmad Faizal Abdull
- Abstract
Introduction: This study was carried out to investigate the phytochemicals and cytotoxicity of M. oleifera seed extracts on Human breast adenocarcinoma cell line (MCF-7), Human hepatocellular carcinoma cell line (Hep G2) and two Human prostate carcinoma cell lines (PC-3 and DU 145). Methods: The seeds were soaked overnight to defat and subsequently extracted in water, hexane, chloroform, acetone, methanol and ethanol followed by phytochemical screening and cytotoxicity in various cancer cells. Results: Crude water extract yielded the highest amount of crude (21.78%), followed by methanol (11.79%), hexane (5.33%), ethanol (1.28%), chloroform (0.71%) and acetone (0.51%) (w/w). The phytochemicals screening showed the presence of saponin, coumarin, alkaloid, quinone and fat in several crude extracts. As for cytotoxicity assay, the crude extracts were tested on breast, liver and two prostate cancer cell lines. After 72 hour of treatment with the crude extracts, MTT assay were done to determine the IC50 value. Crude water extract showed the lowest IC50 value on MCF-7 (18.60 µg/mL) and DU 145 (18.24 µg/mL) and PC-3 (4.12 µg/mL). Crude chloroform and acetone extracts also showed low IC50 value on DU 145 cell line with 19.55 µg/mL and 19.33 µg/mL, respectively. In addition, chloroform extract showed the lowest IC50 value on Hep G2 (16.61 µg/mL). Conclusion: In this study, crude water, chloroform and acetone extracts of M. oleifera seed were found to be active against breast, liver and prostate cancer cell lines. It can be concluded that the extracts from M. oleifera seeds have a great potential for breast, liver and prostate cancer treatment.
- Subjects
LIVER cancer; CELL lines; PROSTATE cancer; CANCER cells; BREAST; COUMARINS
- Publication
Malaysian Journal of Medicine & Health Sciences, 2017, Vol 13, p35
- ISSN
1675-8544
- Publication type
Article