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- Title
Occurrence of coliforms, E. coli type 1 and pathogenic serovar O157:H7 in Iranian burgers.
- Authors
Maktabi, Siavash; Ahangari, Zohreh; Pour, Horyeh Mohammad
- Abstract
Escherichia (E.) coli is an important microbiological sanitary indicator and O157:H7 is one of its serious pathogenic serovars in human. Based on an amendment issued by Iranian National Standards Organization at 2010, the acceptable level for presence of E. coli in raw frozen burgers was removed and producers allowed to made burgers with no obligation in this regard. This study investigated the prevalence and numbers of total coliforms, E. coli Type 1 and serovar O157:H7 in Iranian beef burgers which will be useful in a national monitoring program. Over a 6-month period, 200 frozen burger samples were purchased from 12 Iranian brands. All samples were subjected to MPN and direct plating on VRBA and subsequent biochemical tests for enumeration of coliforms and identification of typical E. coli. In addition, the samples were enriched in TSB with novobiocin and plating on CT-SMAC and finally confirmed by PCR to identify pathogenic serovar O157:H7. In addition, direct PCR assay was performed on all samples to identify possible target genes. E. coli Type1 was recovered from 32 samples with counts over 5x10³ cfu/g and total coliforms from 56 samples with more than 5x10³ cfu/g. E. coli O157:H7 was recovered from 1 sample (0.5%). Our data showed that some products may be unacceptable for consumption. It is recommended that based on national circumstances, the minimum allowable level of E. coli in burgers should be specified by the government authority to ensure public health. Also, a zero tolerance policies for E. coli O157 in meat products could be useful.
- Subjects
COLIFORMS; ESCHERICHIA coli; PATHOGENIC microorganisms; HAMBURGERS; NOVOBIOCIN; POLYMERASE chain reaction
- Publication
International Journal of Pharmaceutical Research & Allied Sciences, 2016, Vol 5, Issue 2, p349
- ISSN
2277-3657
- Publication type
Article