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- Title
Langerhans cell induced cytotoxic T-cell responses against normal human epidermal cell targets: in vitro studies.
- Authors
Faure, M.; Dezutter-Dambuyant, C.; Schmitt, D.; Gaucherand, M.; Thivolet, J.
- Abstract
We investigated the role of class II MHC (HLA-DR Ia-like) antigen-bearing Langerhans cells in the in vitro generation of human alloreactive cytotoxic T lymphocytes (CTL) directed against epidermal cells (BC). Normal BC in suspensions (from reconstructive surgery specimens) and anti-DR monoclonal antibody plus complement (C')-treated BC were used to stimulate peripheral blood (PB) T cells (T cells allogeneic or autologous to EC) in a classical 6-day human mixed skin cell-lymphocyte culture reaction (MSLR). CTL responses were then tested in 51Cr release assays against cultured BC (BC grown on collagen-coated plates in parallel to MSLR). CTL responses to EC were observed only after allogeneic MSLR and when targets and BC used in MSLR were from the same donor. They were comparable in magnitude to those seen in parallel studies using PBL from the same donor as the stimulating BC as target cells. They were abolished when BC used in MSLR were depleted in class II MHC Langerhans cells (preincubation of EC suspensions with anti-DR + C'). These data show: (a) alloreactive CTL to human normal BC may be generated in MSLR, as previously shown in animals; (b) the MSLR is an in vitro model of primary immunization against EC; (c) Langerhans cells are necessary for the generation of cell-mediated cytotoxicity reactions occurring against epidermal cells.
- Subjects
LANGERHANS cells; ANTIGENS; LYMPHOCYTES; T cells; DENDRITIC cells; MONOCLONAL antibodies; EXTRACELLULAR matrix proteins
- Publication
British Journal of Dermatology, 1985, Vol 113, p114
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.1985.tb15638.x