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- Title
Functional and phenotypic analysis of isolated human Langerhans cells and indeterminate cells.
- Authors
Czernielewski, J. M.; Schmitt, D.; Faure, M. R.; Thivolet, J.
- Abstract
In the mixed skin cell lymphocyte culture reaction (MSLR), epidermal cells induce the proliferation <em>in vitro</em> of peripheral blood lymphocytes. Using monoclonal antibodies (MCAB) 0KT6 specific for Langerhans cells among epidermal cells and an anti HLA-Dr, we analysed which epidermal cells are necessary in MSLR in man. Inhibition and reduction of the proliferative responses in MSLR conducted respectively with 'anti HLA-Dr and complement' and '0KT6 and complement'-pretreatcd epidermal cells suggested that not only Langerhans cells but also other HLA-Dr bearing cells play a major role. Immuno-electron microscopic studies of either HLA-Dr ( + ) or OKT6 ( + ) enriched and depleted epidermal cells suspensions: (1) were well correlated with results in MSLR; (2) indicated that Langerhans cells express both T6 and HLA-Dr determinants while indeterminate cells express only the HLA-Dr. No other epidermal cells were found to be either OKT6 or HLA-Dr ( + ). The data strongly suggest that not only Langerhans cells but also OKT6 ( - ) HLA-Dr ( + ) indeterminate cells play a major role in lympho-epidermal interactions.
- Subjects
LANGERHANS cells; PHENOTYPES; EPIDERMIS; LYMPHOCYTES; MONOCLONAL antibodies; CELL culture
- Publication
British Journal of Dermatology, 1983, Vol 108, Issue 2, p129
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.1983.tb00054.x