We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Chemical Study on Protective Effect Against Hydroxyl-induced DNA Damage and Antioxidant Mechanism of Myricitrin.
- Authors
Li, Xican; Mai, Wenqiong; Chen, Dongfeng
- Abstract
Excessive reactive oxygen species (ROS) can oxidatively damage DNA to cause severe biological consequences. In the study, a natural flavonoid, myricitrin (myricetin-3-O-α-L-rhamnopyranoside), was found to have a protective effect against hydroxyl-induced DNA damage (IC50 159.86 ± 54.24 μg/mL). To investigate the mechanism, it was determined by various antioxidant assays. The results revealed that myricitrin could effectively scavenge ·OH, ·O2−, DPPH· (1,1-diphenyl-2-picrylhydrazyl radical), and ABTS+· (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals (IC50 values were respectively 69.71 ± 5.93, 69.71 ± 5.93, 25.34 ± 2.14, and 1.71 ± 0.09 μg/mL), and bind Cu2+ (IC50 27.33 ± 2.36 μg/mL). Based on the mechanistic analysis, it can be concluded that: ( i) myricitrin can effectively protect against hydroxyl-induced DNA oxidative damage via ROS scavenging and deoxynucleotide radicals repairing approaches. Both approaches can be attributed to its antioxidant. From a structure-activity relationship viewpoint, its antioxidant ability can be attributed to the ortho-dihydroxyl moiety, and ultimately to the stability of its oxidized form ortho-benzoquinone; ( ii) its ROS scavenging is mediated via metal-chelating, and direct radical-scavenging which is through donating hydrogen (H·) and electron ( e); and ( iii) its protective effect against DNA oxidative damage may be primarily responsible for the pharmacological effects, and offers promise as a new therapeutic reagent for diseases from DNA oxidative damage.
- Subjects
DNA damage; REACTIVE oxygen species; FLAVONOIDS; ANTIOXIDANTS; HYDROGEN
- Publication
Journal of the Chinese Chemical Society, 2014, Vol 61, Issue 3, p383
- ISSN
0009-4536
- Publication type
Article
- DOI
10.1002/jccs.201300396