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- Title
Experimental selection of long-term intracellular mycobacteria.
- Authors
Vázquez, Cristina L.; Lerner, Thomas R.; Kasmapour, Bahram; Pei, Gang; Gronow, Achim; Bianco, Maria V.; Blanco, Federico C.; Bleck, Christopher K. E.; Geffers, Robert; Bigi, Fabiana; Abraham, Wolf‐Rainer; Gutierrez, Maximiliano G.
- Abstract
Some intracellular bacteria are known to cause long-term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long-term intracellular lifestyle. Selected M ycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids ( PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re-infection and also with the specific expression of stress- and survival-related genes. Our findings identify bacterial traits implicated in the establishment of long-term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
- Subjects
INTRACELLULAR membranes; HOSTS (Biology); BACTERIAL diseases; BIOLOGICAL adaptation; MYCOBACTERIUM bovis; BCG vaccines; GLYCOLIPIDS
- Publication
Cellular Microbiology, 2014, Vol 16, Issue 9, p1425
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/cmi.12303