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- Title
Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface.
- Authors
Seymour, Lisa M.; Jenkins, Cheryl; Deutscher, Ania T.; Raymond, Benjamin B. A.; Padula, Matthew P.; Tacchi, Jessica L.; Bogema, Daniel R.; Eamens, Graeme J.; Woolley, Lauren K.; Dixon, Nicholas E.; Walker, Mark J.; Djordjevic, Steven P.
- Abstract
Summary Mycoplasma hyopneumoniae is a major, economically damaging respiratory pathogen. Although M. hyopneumoniae cells bind plasminogen, the identification of plasminogen-binding surface proteins and the biological ramifications of acquiring plasminogen requires further investigation. mhp182 encodes a highly expressed 102 kDa protein (P102) that undergoes proteolytic processing to generate surface-located N-terminal 60 kDa (P60) and C-terminal 42 kDa (P42) proteins of unknown function. We show that recombinant P102 (rP102) binds plasminogen at physiologically relevant concentrations ( KD ∼ 76 nM) increasing the susceptibility of plasmin(ogen) to activation by tissue-specific plasminogen activator (tPA). Recombinant proteins constructed to mimic P60 (rP60) and P42 (rP42) also bound plasminogen at physiologically significant levels. M. hyopneumoniae surface-bound plasminogen was activated by tPA and is able to degrade fibrinogen, demonstrating the biological functionality of M. hyopneumoniae-bound plasmin(ogen) upon activation. Plasmin(ogen) was readily detected in porcine ciliated airways and plasmin levels were consistently higher in bronchoalveolar lavage fluid from M. hyopneumoniae-infected animals. Additionally, rP102 and rP42 bind fibronectin with KDs of 26 and 33 nM respectively and recombinant P102 proteins promote adherence to porcine kidney epithelial-like cells. The multifunctional binding ability of P102 and activation of M. hyopneumoniae-sequestered plasmin(ogen) by an exogenous activator suggests P102 plays an important role in virulence.
- Subjects
FIBRONECTINS; PLASMIN; MYCOPLASMA; BACTERIAL cell surfaces; BACTERIAL proteins; RECOMBINANT proteins; MICROBIAL virulence
- Publication
Cellular Microbiology, 2012, Vol 14, Issue 1, p81
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/j.1462-5822.2011.01702.x