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- Title
Bartonella henselaeinhibits apoptosis in Mono Mac 6 cells.
- Authors
Kempf, Volkhard A. J.; Schairer, Annette; Neumann, Diana; Grassl, Guntram A.; Lauber, Kirsten; Lebiedziejewski, Maria; Schaller, Martin; Kyme, Pierre; Wesselborg, Sebastian; Autenrieth, Ingo B.
- Abstract
Bartonella henselaecauses the vasculoproliferative disorders bacillary angiomatosis and peliosis probably resulting from the release of vasculoendothelial growth factor (VEGF) from infected epithelial or monocytic host cells. Here we demonstrate thatB. henselaein addition to VEGF induction was also capable of inhibiting the endogenous sucide programme of monocytic host cells. Our results show thatB. henselaeinhibits pyrrolidine dithiocarbamate (PDTC)-induced apoptosis in Mono Mac 6 cells.B. henselaewas observed to be present in a vacuolic compartment of Mono Mac 6 cells. Direct contact ofB. henselaewith Mono Mac 6 cells was crucial for inhibition of apoptosis as shown by the use of a two-chamber model. Inhibition of apoptosis was paralleled by diminished caspase-3 activity which was significantly reduced in PDTC-stimulated andB. henselae-infected cells. The anti-apoptotic effect ofB. henselaewas accompanied by (i) the activation of the transcription factor NF-κB and (ii) the induction of cellular inhibitor of apoptosis proteins-1 and -2 (cIAP-1, -2). Our results suggest a new synergistic mechanism inB. henselaepathogenicity by (i) inhibition of host cell apoptosis via activation of NF-κB and (ii) induction of host cell VEGF secretion.
- Subjects
BARTONELLA; BARTONELLACEAE; APOPTOSIS; CELL death; TRANSCRIPTION factors; CYTOKINES
- Publication
Cellular Microbiology, 2005, Vol 7, Issue 1, p91
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/j.1462-5822.2004.00440.x