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- Title
Salmonella entericaserovar Typhimurium interaction with dendritic cells: impact of thesifAgene.
- Authors
Petrovska, Liljana; Aspinall, Richard J.; Barber, Li; Clare, Simon; Simmons, Cameron P.; Stratford, Richard; Khan, Shahid A.; Lemoine, Nicholas R.; Frankel, Gad; Holden, David W.; Dougan, Gordon
- Abstract
Salmonella entericaserovar Typhimurium (S.Typhimurium) and several mutant derivatives were able to enter efficiently murine bone marrow-derived dendritic cells using mechanisms predominantly independent of the Salmonella pathogenicity island 1 type III secretion system. The levels of intracellular bacteria did not increase significantly over many hours after invasion. Using fluid endocytic tracers and other markers,S.Typhimurium-containing vacuoles (SCVs) were physically distinguishable from early endocytic compartments. Fifty to eighty per cent of SCVs harbouring wild-typeS.Typhimurium oraroA,invHandssaVmutant derivatives were associated with late endosome markers. In contrast,S.TyphimuriumsifAwas shown to escape the SCVs into the cytosol of infected dendritic cells.S.TyphimuriumaroC sifAwas more efficient thanS.TyphimuriumaroCin delivering a eukaryotic promoter-driven green fluorescent protein reporter gene for expression in dendritic cells. In contrast,S.TyphimuriumaroC sifAdid not detectably increase the efficiency of MHC class I presentation of the model antigen ovalbumin to T cells compared to a similararoCderivative. Mice infected with theS.TyphimuriumaroC sifAexpressing ovalbumin did not develop detectably enhanced levels of cytotoxic T cell or interferon-γ production compared toS.TyphimuriumaroCderivatives.
- Subjects
SALMONELLA; ANTIGEN presenting cells; DENDRITIC cells; GENES; LYMPHOCYTES; T cells; MICROBIOLOGY
- Publication
Cellular Microbiology, 2004, Vol 6, Issue 11, p1071
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/j.1462-5822.2004.00419.x