We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The QuantuMDx Q-POC SARS-CoV-2 RT-PCR assay for rapid detection of COVID-19 at point-of-care: preliminary evaluation of a novel technology.
- Authors
Caffry, Jessica; Selby, Matthew; Barr, Katie; Morgan, George; McGurk, David; Scully, Philip; Park, Catherine; Caridis, Anna-Maria; Southworth, Emily; Morrison, Jack; Clark, David J.; Davies, Benedict M. O.; Eckersley, Nicholas M.; Groppelli, Elisabetta; Kirwan, Daniela E.; Monahan, Irene; Augustin, Yolanda; Toombs, Colin; Planche, Tim; Staines, Henry M.
- Abstract
Accurate and rapid point-of-care (PoC) diagnostics are critical to the control of the COVID-19 pandemic. The current standard for accurate diagnosis of SARS-CoV-2 is laboratory-based reverse transcription polymerase chain reaction (RT-PCR) assays. Here, a preliminary prospective performance evaluation of the QuantuMDx Q-POC SARS-CoV-2 RT-PCR assay is reported. Between November 2020 and March 2021, 49 longitudinal combined nose/throat (NT) swabs from 29 individuals hospitalised with RT-PCR confirmed COVID-19 were obtained at St George's Hospital, London. In addition, 101 mid-nasal (MN) swabs were obtained from healthy volunteers in June 2021. These samples were used to evaluate the Q-POC SARS-CoV-2 RT-PCR assay. The primary analysis was to compare the sensitivity and specificity of the Q-POC test against a reference laboratory-based RT-PCR assay. The overall sensitivity of the Q-POC test compared with the reference test was 96.88% (83.78– 99.92% CI) for a cycle threshold (Ct) cut-off value for the reference test of 35 and 80.00% (64.35–90.95% CI) without altering the reference test's Ct cut-off value of 40. The Q-POC test is a sensitive, specific and rapid PoC test for SARS-CoV-2 at a reference Ct cut-off value of 35. The Q-POC test provides an accurate option for RT-PCR at PoC without the need for sample pre-processing and laboratory handling, enabling rapid diagnosis and clinical triage in acute care and other settings.
- Subjects
SAINT George (Utah); LONDON (England); REVERSE transcriptase polymerase chain reaction; TESTING laboratories; SARS-CoV-2; COVID-19; MICROFLUIDICS
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-35479-9