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- Title
Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation.
- Authors
Costantini, Veronica; Vinjé, Jan; Morantz, Esther K.; Browne, Hannah; Ettayebi, Khalil; Xi-Lei Zeng; Atmar, Robert L.; Estes, Mary K.; Zeng, Xi-Lei
- Abstract
Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
- Subjects
NOROVIRUS diseases; VIRUS inactivation; DUODENAL diseases; ENTEROVIRUS diseases; ENTEROCYTES; ALCOHOLS (Chemical class); CELL lines; CHLORINE; COMPARATIVE studies; JEJUNUM; RESEARCH methodology; MEDICAL cooperation; MICROBIOLOGICAL techniques; RESEARCH; RNA viruses; EVALUATION research
- Publication
Emerging Infectious Diseases, 2018, Vol 24, Issue 8, p1453
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2408.180126