We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells.
- Authors
Zhuang, Shaowei; Cheng, Tzu-Hurng; Shih, Nang-Lang; Liu, Ju-Chi; Chen, Jin-Jer; Hong, Hong-Jye; Chan, Paul
- Abstract
Tanshinone IIA is the main effective component of Salvia miltiorrhiza, known as 'Danshen,' which has been used in many therapeutic remedies in traditional Chinese medicine. However, the direct effects of tanshinone IIA on vascular endothelial cells have not yet been fully described. In the present study, we demonstrated that tanshinone IIA increased heme oxygenase-1 (HO-1) expression in human umbilical vein endothelial cells. Western blot analyses and experiments with specific inhibitors indicated tanshinone IIA enhanced HO-1 expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt and the subsequent induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. In addition, tanshinone IIA inhibited cyclic strain induced interleukin-8 (IL-8) expression. HO-1 silencing significantly abrogated the repressive effects of tanshinone IIA on strain-induced IL-8 expression, which suggests HO-1 has a role in mediating the effects of tanshinone IIA. This study reports for the first time that tanshinone IIA inhibits cyclic strain-induced IL-8 expression via the induction of HO-1 in endothelial cells, providing valuable new insight into the molecular pathways that may contribute to the effects of tanshinone IIA.
- Subjects
ANALYSIS of variance; CELL culture; ELECTROPHORESIS; EPITHELIAL cells; INTERLEUKINS; CHINESE medicine; OXIDOREDUCTASES; POLYMERASE chain reaction; PROBABILITY theory; PROTEIN kinases; RESEARCH funding; STATISTICS; WESTERN immunoblotting; PLANT extracts; DATA analysis; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies
- Publication
American Journal of Chinese Medicine, 2016, Vol 44, Issue 2, p377
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X1650021X