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- Title
KNOCK-OUT MUTATION OF CAT2 DRASTICALLY REDUCED THE MELATONIN-MEDIATED RESISTANCE TO BORON TOXICITY IN ARABIDOPSIS THALIANA.
- Authors
SAGLAM, AYKUT; MUSLU, ASIYE SEZGIN; ALTUNTAS, CANSU; DEMIRALAY, MEHMET; KADIOGLU, ASIM
- Abstract
The role of catalase in resistance to boron toxicity after melatonin application (MEL) was investigated in Arabidopsis thaliana plants. Col-0 and cat2-2 plants were exposed to 50 µM MEL followed by boron toxicity (BT) in a medium containing 10 mM H3BO3. Pigment loss and accordingly chlorosis were reduced by melatonin under BT conditions, while they were more prominent in cat2-2 mutants. Moreover, TBARS and H2O2 contents, which increased due to BT, decreased as a result of melatonin application and the levels of these parameters in cat2-2 mutants were higher than the values in Col-0. Antioxidant enzyme activity of SOD and SOD1 gen transcript were induced by MEL under BT. Conversely, APX4, PER10 and CAT1 transcripts were down-regulated by MEL under BT. In addition, antioxidant enzyme activities and their transcript levels were lower than those of Col-0. Thus, we suggested that MEL scavenged ROS directly under BT. Melatonin also reduced the accumulation of boric acid in leaf tissues of Col-0, but not cat2-2. Finally, even though melatonin application provided a degree of endurance, the cat2 mutation resulted in increased sensitivity to BT.
- Publication
Acta Biologica Cracoviensia Series Botanica, 2023, Vol 65, Issue 2, p7
- ISSN
0001-5296
- Publication type
Article
- DOI
10.24425/abcsb.2023.145595