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- Title
SYNTHESIS AND SPECTRAL-FLUORESCENT STUDY OF PROTEIN COATINGS OF MAGNETIC NANOPARTICLES USING CARBOCYANINE DYES.
- Authors
Pronkin, P. G.; Bychkova, A. V.; Sorokina, O. N.; Tatikolov, A. S.; Kovarskii, A. L.; Rosenfeld, M. A.
- Abstract
Formation of the biocompatible coatings on the surface of magnetic nanoparticles (MNPs) opens up prospects for their use in medicine. In this work, magnetite nanoparticles have been obtained with stable coatings formed from bovine and human serum albumins by free radical cross-linking (Fenton reaction). We studied the interaction of anionic carbocyanine dyes with bovine and human serum albumins (BSA and HSA) composed of the coatings on magnetic nanoparticles. Electronic excitation energy transfer (EEET) between molecules of carbocyanine dyes has been studied both in samples of HSA solutions and in systems containing BSA/HSA coated MNPs. The effect of HSA denaturation on the spectral and fluorescent properties of the dyes and EEET has been studied, and the data on donor fluorescence quenching by the acceptor have been obtained. It has been found that ~ 90% of albumin molecules forming the MNPs coatings retain their capability of binding with a fluorescent dye. Albumins were shown to retain their functional properties in the cross-linked coating, thereby providing evidence for biocompatibility of the coatings obtained by free radical oxidative protein modification. Carbocyanine dyes are suggested as spectralfluorescent probes for assessment of the functional properties of the albumin molecules forming the MNPs coatings.
- Subjects
MAGNETITE; MAGNETIC nanoparticles; SERUM albumin; HABER-Weiss reaction; CYANINES; ENERGY transfer; BIOCOMPATIBILITY
- Publication
Journal of Nature Science & Sustainable Technology, 2015, Vol 9, Issue 3, p645
- ISSN
1933-0324
- Publication type
Article