We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytoplasmic zinc promotes IL-1β production by monocytes and macrophages through mTORC1-induced glycolysis in rheumatoid arthritis.
- Authors
Kim, Bonah; Kim, Hee Young; Yoon, Bo Ruem; Yeo, Jina; In Jung, Ji; Yu, Kyung-Sang; Kim, Hyeon Chang; Yoo, Su-Jin; Park, Jin Kyun; Kang, Seong Wook; Lee, Won-Woo
- Abstract
In addition, LPS stimulation led to an increase of I Zip8 i in both monocytes and macrophages, suggesting that Zip8 plays important roles in the regulation of zinc influx in monocytes and macrophages. Increased extracellular zinc boosts production of IL-1 in human monocytes/macrophages.(A and B) Freshly purified monocytes from healthy donors (A) or monocyte-derived macrophages (B) were incubated with the zinc chelator TPEN (150 nM) or the indicated concentration of ZnCl 2 for 2 hours and stimulated with LPS for 24 hours. Enhanced expression of zinc transporter Zip8 in monocytes of patients with RA and activated monocytes/macrophages of healthy controls.(A) Microarray analysis on 10 SLC30A (ZnT) and 14 SLC39A (Zip) transporters expressed by peripheral monocytes. Given that T cell activation induces changes in the expression or activity of many zinc transporters ([32]), we first assessed the expression of I Zip i genes in monocytes and monocyte-derived macrophages (hereafter, macrophages) upon LPS stimulation. Cytoplasmic zinc promotes IL-1 production by monocytes and macrophages through mTORC1-induced glycolysis in rheumatoid arthritis.
- Subjects
MONOCYTES; GLYCOLYSIS; RHEUMATOID arthritis; MACROPHAGES; ZINC
- Publication
Science Signaling, 2022, Vol 15, Issue 716, p1
- ISSN
1945-0877
- Publication type
Article
- DOI
10.1126/scisignal.abi7400