We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential Modulation of Circulating Endothelial Progenitor Cells in Peripheral Blood Monocyte Population of an Early Stage of Streptozotocin-Induced Diabetic Retinopathy.
- Authors
Rupadevi, Muthaian; Raveendran, Ramasamy
- Abstract
Objective: To enumerate the circulating endothelial progenitor cells (EPCs) in peripheral blood mononuclear cells (PBMC) in streptozotocin (STZ) induced diabetic retinopathy (DR) rat model. Materials and Methods: Retinal microvascular complications in STZ induced diabetic Wistar rats were validated after 4 months using trypsin digestion assay of retinal vasculature, histological analysis of retina, glial fibrillary acidic protein (GFAP) immunostaining. Subsequently, circulating EPCs were quantified in PBMCs from diabetes induced rats after 4 months using markers such as CD-34, CD-31, CD-146, VE-Cadherin and Flk-1 by flow cytometry. Results: Pericyte loss, acellular capillaries, retinal layer thickness, microglial activation and increased nitric oxide (NO) levels were observed after 4 months of diabetes induction. Whereas the EPC markers. While the EPC markers such as CD-34, CD-31, VE-Cadherin were decreased, the Flk-1+ve cells were up-regulated in the endogenous EPC pool. Conclusion: The quantity of EPCs is modulated in diabetes which may provide an insight into the use of EPCs as a biomarker for the progression of diabetes induced microvascular complication.
- Subjects
PROGENITOR cells; CYTOPROTECTION; HUMAN ecology; STREPTOZOTOCIN; NITROSOUREAS
- Publication
Journal of Young Pharmacists, 2016, Vol 8, Issue 3, p198
- ISSN
0975-1483
- Publication type
Article
- DOI
10.5530/jyp.2016.3.6