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- Title
Isolation and screening of Lactobacillus species from dogs for probiotic action.
- Authors
Mohammad Sharif, N.; Sreedevi, B.; Chaitanya, R. K.; Sreenivasulu, D.
- Abstract
The present study aimed at the detection of suitable Lactobacillus species from dogs for usage as probiotic. A total of 67 rectal swabs from healthy pups were analyzed and 49 (73.1%) Lactobacillus isolates were identified based on morphological, biochemical characteristics and confirmed by genus specific PCR targeting 16S rRNA gene. A total of 20 isolates that showed strong aggregation, high cell surface hydrophobicity, acid and bile tolerance were screened for in-vitro antibacterial activity by agar well diffusion assay, where prominent inhibitory zones were observed against majority of the test pathogens. Reduction in antibacterial activity was noticed after neutralization, proteinase K and heat treatment of supernatants. Partial 16S rRNA nucleotide sequence analysis of seven Lactobacillus isolates that showed prominent in-vitro antibacterial activity revealed maximum sequence homology with Lactobacillus fermentum (for six isolates) and Lactobacillus agilis (for one isolate). The in-vitro antibacterial activity results highlighted the need for in-vivo studies in India to establish probiotic potential of canine faecal Lactobacillus species in the near future.
- Subjects
LACTOBACILLUS; PROBIOTICS; LACTOBACILLUS rhamnosus; ESCHERICHIA coli; ANIMAL nutrition
- Publication
Indian Journal of Animal Research, 2018, Vol 52, Issue 12, p1739
- ISSN
0367-6722
- Publication type
Article
- DOI
10.18805/ijar.B-3429