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- Title
Alpha-6 (CD 49f) integrin expression in genetic and acquired bullous skin diseases: A comparison of its distribution with bullous pemphigoid antigen.
- Authors
Kanitakis, J.; Zambruno, G.; Vassileva, S.; Giannetti, A.; Thivolet, J.
- Abstract
Bullous pemphigoid (BP) antigen and α6 integrin are hernidesmosome-associated glycoproteins of basal keratinocytes. In this work, the immunoreactivity of antibodies to BP and to &alpha6 in salt- or dispase-split human skin, and in 4(3 biopsy specimens of various genetic and autoimmune bullous dermatoses taken from various body sites, was studied by double-labeling immunofluorescence. In all specimens, both antigens localized at the same side of the blisters observed, i.e. the roof of the bulla in cases with a junctional or dermolytic cleavage, or the floor of the blister in those with intracpidermal cleavage. Immunostaining for α6 was strong and present in all specimens studied, whereas the one obtained with the BP serum was absent from some specimens. These results show that the BP antigen and the α6 integrin colocalize at the level of cleavage in bullous diseases; however, the more consistent and reproducible reactivity obtained with the anti-α6 antibody suggests that this should the preferentially used in the immunohistochemical investigation of bullous dermatoses.
- Subjects
GLYCOPROTEINS; KERATINOCYTES; ANTIGENS; IMMUNOGLOBULINS; SKIN diseases; IMMUNOFLUORESCENCE
- Publication
Journal of Cutaneous Pathology, 1992, Vol 19, Issue 5, p376
- ISSN
0303-6987
- Publication type
Article
- DOI
10.1111/j.1600-0560.1992.tb00609.x