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- Title
Molecular characteristics of an immobilization antigen gene of the fish-parasitic protozoan Ichthyophthirius multifiliis strain ARS-6.
- Authors
De-Hai Xu; Panangala, Victor S.; van Santen, Vicky L.; Dybvig, Kevin; Abernathy, Jason W.; Klesius, Phillip H.; Zhanjiang Liu; Russo, Riccardo
- Abstract
Ichthyophthirius multifiliis (Ich), a ciliated protozoan parasite of fish, expresses surface antigens (i-antigens), which react with host antibodies that render them immobile. The nucleotide sequence of an i-antigen gene of I. multifiliis strain ARS-6 was deduced. The predicted protein of 47 493 Da is comprised of 460 amino acids (aa's) arranged into five imperfect repeats with periodic cysteine residues with the structure: CX(19)20CX2CX16−27CX2CX20(21)CX3. The N-terminal aa's typify a signal peptide motif while a stretch of C-terminal aa's resemble a glycosyl–phosphatidyl–inositol (GPI)-anchor addition site. The degree of deduced i-antigen aa sequence identity of strain ARS-6 (GenBank accession # ACH87654 and # ACH95659) with other I. multifiliis i-antigen sequences present in GenBank ranges from 99% to 36% identity with 52 kDa i-antigens of I. multifiliis strain G5 (accession #s AAK94941 and AAK01661 respectively). Immunoblot analysis of i-antigens following exposure of I. multifiliis theronts to catfish anti- I. multifiliis immune serum did not show any appreciable alteration in i-antigen expression. The mechanism that regulates i-antigen expression in I. multifiliis remains a puzzling question.
- Subjects
ICHTHYOPHTHIRIUS multifiliis; CELL surface antigens; IMMUNOGLOBULINS; NUCLEOTIDE sequence; AMINO acids
- Publication
Aquaculture Research, 2009, Vol 40, Issue 16, p1884
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/j.1365-2109.2009.02301.x