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- Title
Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK
- Authors
Vorderwülbecke, S.; Kramer, G.; Merz, F.; Kurz, T.A.; Rauch, T.; Zachmann-Brand, B.; Bukau, B.; Deuerling, E.
- Abstract
Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60 kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.
- Subjects
ESCHERICHIA coli; PROTEIN folding; CELL aggregation; MOLECULAR chaperones
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p181
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00052-3