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- Title
Regulation of flotillin-1 in the establishment of NIH-3T3 cell–cell interactions<sup>1</sup><FN ID="FN1"><NO>1</NO>The nucleotide sequence data reported are available in the GenBank database under accession numbers AY167925 and AY168443.</FN>
- Authors
López-Casas, Pedro P.; del Mazo, Jesús
- Abstract
Flotillin-1 is a lipid raft-associated protein involved in neuronal regeneration, insulin signaling in adipocytes, and phagosome maturation in macrophages. We detected abundant flotillin-1 mRNA expression in confluent cultures of mouse NIH-3T3 fibroblasts. These cells were used as a model to assess the regulation of flotillin-1 mRNA and protein expression by cell–cell interactions. The disruption of cell–cell interactions triggered the downregulation of the flotillin-1 gene. However, the establishment of new cell interactions, and finally confluence, increased both the flotillin-1 transcript and its protein. Despite flotillin-1 having been described as an integral protein of the cell membrane, it is also very conspicuous in the hydrophilic fraction (the non-membrane fraction) of confluent cell extracts. The promoter region of the flotillin-1 gene was isolated and analyzed by transfection experiments, and the regulatory domains containing motifs for binding transcription factors involved in cell differentiation were identified.
- Subjects
LIPIDS; PROTEINS; NEURONS; MESSENGER RNA
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p223
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01239-0