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- Title
Akt signaling mediates VEGF/VPF vascular permeability in vivo
- Authors
Six, Isabelle; Kureishi, Yasuko; Luo, Zhengyu; Walsh, Kenneth
- Abstract
VEGF is an endothelial cell cytokine that promotes angiogenesis and enhances microvascular permeability. Recently, it has been shown that the protein kinase Akt functions in a key intercellular signaling pathway downstream of VEGF. Here, we employed adenovirus-mediated gene transfer in conjunction with the Miles assay in hairless albino guinea pigs to assess the role of Akt signaling in vascular permeability. VEGF-induced vascular permeability was blocked by the transduction of a dominant negative mutant of Akt. Conversely, transduction of a constitutively active form of Akt promoted vascular permeability in a manner similar to VEGF protein administration. This Akt-mediated increase in vascular permeability was inhibited by the eNOS inhibitor L-NAME. These data show that Akt signaling is both necessary and sufficient for vascular permeability in an in vivo model.
- Subjects
VASCULAR endothelium; PROTEIN kinases
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p67
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03630-X