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- Title
Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis.
- Authors
Nikolova, Biliana; Kostadinova, Anelia; Dimitrov, Borislav; Zhelev, Zhivko; Bakalova, Rumiana; Aoki, Ichio; Saga, Tsuneo; Tsoneva, Iana
- Abstract
The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions' interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.
- Subjects
CANCER chemotherapy; KERATINOCYTES; CELL lines; T cells; RIFAMPIN; PSORIASIS; BIOSENSORS
- Publication
Sensors (14248220), 2013, Vol 13, Issue 3, p3625
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s130303625