We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Surface densities of ephrin-B1 determine EphB1-coupled activation of cell attachment through a<sub>v</sub>ß<sub>3</sub> and a<sub>5</sub>ß<sub>1</sub> integrins.
- Authors
Uyen Huynh-Do; Stein, Elke; Lane, Andy A.; Hua Liu; Cerretti, Douglas P.; Daniel, Thomas O.
- Abstract
Receptors of the Eph family and their ligands (ephrins) mediate developmental vascular assembly and direct axonal guidance. Migrating cell processes identify appropriate targets within migratory fields based on topographically displayed ephrin gradients. Here, EphB1 regulated cell attachment by discriminating the density at which ephrin-B1 was displayed on a reconstituted surface. EphB1—ephrin-B1 engagement did not promote cell attachment through mechanical tethering, but did activate integrin-mediated attachment. In endothelial cells, attachment to RGD peptides or fibrinogen was mediated through ανβ3 integrin. EphB1 transfection conferred ephrin-B1-responsive activation of α5β1 integrin-mediated cell attachment in human embryonic kidney cells. Activation-competent but signaling-defective EphB1 point mutants failed to stimulate ephrin-B1 dependent attachment. These findings lead us to propose that EphB1 functions as a ‘ligand density sensor’ to signal integrin-mediated cell—matrix attachment.
- Subjects
CELL receptors; LIGANDS (Biochemistry); BLOOD-vessel development; AXONS; CELL migration; FIBRINOGEN; INTEGRINS; HUMAN embryos
- Publication
EMBO Journal, 1999, Vol 18, Issue 8, p2165
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/18.8.2165