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- Title
A silanized mica substrate suitable for high-resolution fiber FISH analysis by scanning near-field optical/atomic force microscopy.
- Authors
Sugiyama, Shigeru; Fukuta, Megumi; Hirose, Tamaki; Ohtani, Toshio; Yoshino, Tomoyuki
- Abstract
We applied a novel silanized mica substrate with an extremely flat surface constructed according to Sasou et al. ( Langmuir , 9845-9849 (2003)) to high-resolution detection of a specific gene on a DNA fiber by scanning near-field optical/atomic force microscopy (SNOM/AFM). The interaction between the substrate and fluorescence-dye conjugated peptide nucleic acid (PNA) probes, which causes fluorescence noise signal, was minimal. By using the substrate, we successfully obtained a fluorescence in situ hybridization signal from the ea47 gene on a λphage DNA labeled with an Alexa 532-conjugated 15-base PNA probe. As the results, no fluorescence noises were observed, indicating that the surface adsorbed almost none of the PNA probe. The combination of the substrate and SNOM/AFM is an effective tool for visualizing DNA sequences at nanometer-scale resolution. SCANNING 32: 383-389, 2010. © 2010 Wiley Periodicals, Inc.
- Publication
Scanning, 2010, Vol 32, Issue 6, p383
- ISSN
0161-0457
- Publication type
Article
- DOI
10.1002/sca.20214