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- Title
Morphologic variations in bacteria under stress conditions: Near- field optical studies.
- Authors
Cefalì, E.; Patanè, S.; Arena, A.; Saitta, G.; Guglielmino, S.; Cappello, S.; Nicolò, M.; Allegrini, M.
- Abstract
The morphologic and structural variations suffered by cells of a population of Pseudomonas aeruginosa ATCC 27853 under stress conditions were investigated by using scanning near-field optical microscopy. The analysis of the images, supported by microbiological data, showed that the bacteria evolved from the initial distribution of rod-shaped cells of standard size to a population with structural and morphologic modifications. The detection of variations in the optical reflectivity over a sub-wavelength scale (⩽ 100 nm), combined with the concurrently acquired topographical signal, allowed the visualisation of rod-shaped bacteria going towards a lytic process and entire 'U'-shaped cells. In the latter cells, which derived from a morphology refolding of rod bacteria, cellular matter seemed to rearrange itself to attain a coccoid stress resistant form, responsible for the residual viability of the population.
- Publication
Scanning, 2002, Vol 24, Issue 6, p274
- ISSN
0161-0457
- Publication type
Article
- DOI
10.1002/sca.4950240601