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- Title
Small Animal Imaging: The Use of Pet/ct Fusion to In Vivo and Longitudinally Assess Anti-Obesity Drug Effects on Fdg Uptake in Mice Target Tissues.
- Authors
Bucci, Marco; Quarta, Carmelo; Nanni, Cristina; Cervino, Cristina; Vicennati, Valentina; Gambineri, Alessandra; Iozzo, Patricia; Nuutila, Pirjo; Fanti, Stefano; Pasquali, Renato; Pagotto, Uberto
- Abstract
FDG and PET have rapidly gained importance in the preclinical studies on control of energy metabolism. Here we propose the combined use of microPET and microCT in obese rodents undergoing longitudinal evaluations during nutritional and pharmacological intervention. Two groups of Ob/Ob mice were on high fat diet (HFD) for 10 weeks and treated with placebo or with CL316243, a selective agonist of beta 3 adrenergic receptor (0.1 mg/kg daily for the last. 10 days of the HFD). The PET/CT scans were performed before and after treatment as follows: the injection of 25 MBq FDG was performed i.v. while mice were under gas anaesthesia; after 1 hour uptake, animals were placed on the translating bed of the machine (microPET Explore Vista by GE). Two separate instruments were used (microPET and microCT) and great care was taken to detect for both microPET and microCT the position of reference points to co-register the two images by software. The PET scan was performed in Static Mode for 30 min = 15 min x 2 beds/FOVs (about 4 mm each). After the scan animals were moved to the second machine (microCT Explore Locus by GE) and CT scan was performed. The post-processing of images has been performed by AMIDE Software. This allows us to co-register the two images and draw ROIs on the PET image according to the CT reference. ROIs were traced in the myocardium, liver, white (WAT) and brown adipose tissues (BAT). Left ventricular cavity of the heart has been selected as reference background area. The myocardium FDG uptake and its TBR were the highest in the whole body in all the experimental setting, followed by the brown adipose tissue (BAT), the liver and the white adipose tissue (WAT). Beta 3 agonist treatment has been shown to selectively work on BAT (P = 0,055), whereas the myocardium, the liver and the WAT do not show significant variations between pre-treatment and post-treatment scans. This novel approach (MicroPET/CT) may improve our knowledge on metabolic processes on small animals by helping to evaluate the target tissues, which were previously difficult to identify by using PET alone. Moreover, our data indicate that the combined PET/CT approach will facilitate the understanding of the response to treatment, allowing repeated longitudinal evaluations in the same animal during nutritional or drug interventions.
- Subjects
SCANNING systems; IMAGING systems; APPETITE depressants; DRUG efficacy; ADRENERGIC receptors; ADIPOSE tissues; LABORATORY mice
- Publication
Diabetes, 2007, Vol 56, pA460
- ISSN
0012-1797
- Publication type
Article