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- Title
miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway.
- Authors
Qin, Chunyu; Wang, Hui; Zhong, Jincheng; Ran, Hongbiao; Peng, Wei
- Abstract
miR-129 plays a crucial role in regulating various cellular processes, including adipogenesis; however, its downstream molecular mechanisms remain unclear. In this study, we demonstrated that miR-129 promotes yak adipogenesis in vitro via the PI3K/AKT pathway. Overexpression and interference of miR-129 in yak intramuscular preadipocytes (YIMAs) enhanced and inhibited cell differentiation, respectively, with corresponding changes in cell proliferation. Further investigation revealed that miR-129 enhances AKT and p-AKT activity in the AKT pathway without affecting cell apoptosis, and a specific inhibitor (LY294002) was used to confirm that miR-129 regulates YIMAs proliferation and differentiation through the PI3K/AKT pathway. Our findings suggest that miR-129 promotes yak adipogenesis by enhancing PI3K/AKT pathway activity. This study provides the foundation to precisely elucidate the molecular mechanism of miR-129 in YIMAs adipogenesis and develop advanced miRNA-based strategies to improve meat nutrition and obesity-related ailments in beef production.
- Subjects
ADIPOGENESIS; PI3K/AKT pathway; YAK; BEEF industry; CELL differentiation; CELL proliferation
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 1, p632
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25010632