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- Title
Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus.
- Authors
Asins, Maria J.; Bullones, Amanda; Raga, Veronica; Romero-Aranda, Maria R.; Espinosa, Jesus; Triviño, Juan C.; Bernet, Guillermo P.; Traverso, Jose A.; Carbonell, Emilio A.; Claros, M. Gonzalo; Belver, Andres
- Abstract
The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided.
- Subjects
CLEOPATRA, Queen of Egypt, d. 30 B.C.; ROOTSTOCKS; LOCUS (Genetics); CITRUS; TRANSCRIPTOMES; GENE expression; GENES
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 21, p15759
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms242115759