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- Title
Endoplasmic Reticulum and Mitochondria Contacts Correlate with the Presence and Severity of NASH in Humans.
- Authors
Jin, Chaonan; Felli, Eric; Lange, Naomi Franziska; Berzigotti, Annalisa; Gracia-Sancho, Jordi; Dufour, Jean-François
- Abstract
The interaction between the mitochondria and the endoplasmic reticulum (ER) is essential for hepatocyte function. An increase in ER–mitochondria contacts (ERMCs) is associated with various metabolic diseases. Non-alcoholic fatty liver disease (NAFLD) is associated with obesity and type 2 diabetes, and its progressive form non-alcoholic steatohepatitis (NASH) can lead to cirrhosis and hepatocellular carcinoma. However, the role of ERMCs in the progression of NAFL to NASH is still unclear. We assessed whether ERMCs could correlate with NAFLD severity. We used a proximity ligation assay to measure the abundance of ERMCs in liver biopsies from patients with biopsy-proven NAFLD (n = 48) and correlated the results with histological and metabolic syndrome (MetS) features. NAFLD patients were included according to inclusion and exclusion criteria, and then assigned to NAFL (n = 9) and NASH (n = 39) groups. ERMCs density could discriminate NASH from NAFL (sensitivity 61.5%, specificity 100%). ERMCs abundance correlated with hepatocellular ballooning. Moreover, the density of ERMCs increased with an increase in the number of MetS features. In conclusion, ERMCs increased from NAFL to NASH, in parallel with the number of MetS features, supporting a role for this interaction in the pathophysiology of NASH.
- Subjects
HELLP syndrome; NON-alcoholic fatty liver disease; MITOCHONDRIA; ENDOPLASMIC reticulum; LIVER histology; TYPE 2 diabetes
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 15, p8348
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23158348