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- Title
Osteoarthritis-Related Inflammation Blocks TGF-β's Protective Effect on Chondrocyte Hypertrophy via (de)Phosphorylation of the SMAD2/3 Linker Region.
- Authors
Thielen, Nathalie; Neefjes, Margot; Wiegertjes, Renske; van den Akker, Guus; Vitters, Elly; van Beuningen, Henk; Blaney Davidson, Esmeralda; Koenders, Marije; van Lent, Peter; van de Loo, Fons; van Caam, Arjan; van der Kraan, Peter
- Abstract
Osteoarthritis (OA) is a degenerative joint disease characterized by irreversible cartilage damage, inflammation and altered chondrocyte phenotype. Transforming growth factor-β (TGF-β) signaling via SMAD2/3 is crucial for blocking hypertrophy. The post-translational modifications of these SMAD proteins in the linker domain regulate their function and these can be triggered by inflammation through the activation of kinases or phosphatases. Therefore, we investigated if OA-related inflammation affects TGF-β signaling via SMAD2/3 linker-modifications in chondrocytes. We found that both Interleukin (IL)-1β and OA-synovium conditioned medium negated SMAD2/3 transcriptional activity in chondrocytes. This inhibition of TGF-β signaling was enhanced if SMAD3 could not be phosphorylated on Ser213 in the linker region and the inhibition by IL-1β was less if the SMAD3 linker could not be phosphorylated at Ser204. Our study shows evidence that inflammation inhibits SMAD2/3 signaling in chondrocytes via SMAD linker (de)-phosphorylation. The involvement of linker region modifications may represent a new therapeutic target for OA.
- Subjects
POST-translational modification; OSTEOARTHRITIS; HYPERTROPHY; INFLAMMATION; PROTEIN domains
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 15, p8124
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22158124