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- Title
Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel.
- Authors
Hao-Che Tang; Wei-Chuan Chen; Chih-Wei Chiang; Lei-Yen Chen; Yu-Ching Chang; Chih-Hwa Chen
- Abstract
This article studied the effects of platelet-rich plasma (PRP) on the potential of synovial fluid mesenchymal stem cells (SF-MSCs) to differentiate. The PRP and SF-MSCs were obtained from the blood and knees of pigs, respectively. The identification of SF-MSCs and their ability to differentiate were studied by histological and surface epitopes, respectively. The SF-MSCs can undergo trilineage mesenchymal differentiation under osteogenic, chondrogenic, and adipocyte induction. The effects of various PRP concentrations (0%, 20% and 50% PRP) on differentiation were evaluated using the SF-MSCs-alginate system, such as gene expression and DNA proliferation. A 50% PRP concentration yielded better differentiation than the 20% PRP concentration. PRP favored the chondrogenesis of SF-MSCs over their osteogenesis in a manner that depended on the ratios of type II collagen/type I collagen and aggrecan/osteopontin. Eventually, PRP promoted the proliferation of SF-MSCs and induced chondrogenic differentiation of SF-MSCs in vitro. Both PRP and SF-MSCs could be feasibly used in regenerative medicine and orthopedic surgeries.
- Subjects
SWINE; REGENERATION (Biology); CYTOLOGY; SYNOVIAL fluid; SYNOVIAL membranes; STEM cell culture; PROGENITOR cells
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 8, p18507
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms160818507