We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans.
- Authors
Dongqin Wang; Yanqun Li; Xueqiong Hu; Weimin Su; Min Zhong
- Abstract
Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydrolysis with high-pressure homogenization and ultrasonication were employed in this study to disrupt the cells of the microalga Neochloris oleoabundans. The cell disruption degree was investigated. The cell morphology before and after disruption was assessed with scanning and transmission electron microscopy. The energy requirements and the operation cost for wet cell disruption were also estimated. The highest disruption degree, up to 95.41%, assessed by accounting method was achieved by the combination of enzymatic hydrolysis and high-pressure homogenization. A lipid recovery of 92.6% was also obtained by the combined process. The combined process was found to be more efficient and economical compared with the individual process.
- Subjects
GREEN algae; LIPIDS; MICROALGAE; BIODIESEL fuels; RENEWABLE energy sources
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 4, p7707
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms16047707