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- Title
UVB-Stimulated TNFa Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK.
- Authors
Muthusamy, Visalini; Piva, Terrence J.
- Abstract
Ultraviolet (UV) radiation activates cell signaling pathways in melanocytes. As a result of altered signaling pathways and UV-induced cellular damage, melanocytes can undergo oncogenesis and develop into melanomas. In this study, we investigated the effect of UV-radiation on p38 MAPK (mitogen-activated protein kinase), JNK and NF?B pathways to determine which plays a major role in stimulating TNFa secretion in human HEM (melanocytes) and MM96L (melanoma) cells. MM96L cells exhibited 3.5-fold higher p38 activity than HEM cells at 5 min following UVA + B radiation and 1.6-fold higher JNK activity at 15-30 min following UVB+A radiation, while NF?B was minimally activated in both cells. Irradiated HEM cells had the greatest fold of TNFa secretion (UVB: 109-fold, UVA + B: 103-fold & UVB+A: 130-fold) when co-exposed to IL1a. The p38 inhibitor, SB202190, inhibited TNFa release by 93% from UVB-irradiated HEM cells. In the UVB-irradiated MM96L cells, both SB202190 and sulfasalazine (NF?B inhibitor) inhibited TNFa release by 52%. Although, anisomycin was a p38 MAPK activator, it inhibited TNFa release in UV-irradiated cells. This suggests that UV-mediated TNFa release may occur via different p38 pathway intermediates compared to those stimulated by anisomycin. As such, further studies into the functional role p38 MAPK plays in regulating TNFa release in UV-irradiated melanocyte-derived cells are warranted.
- Subjects
TUMOR necrosis factors; MELANOCYTES; ULTRAVIOLET radiation; MITOGEN-activated protein kinases; CELLULAR signal transduction; CARCINOGENESIS; ANTIBIOTICS
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 8, p17029
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms140817029