We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells.
- Authors
Chang, Mun-Seog; Kim, Won-Nam; Yang, Woong-Mo; Kim, Hyu-Young; Oh, Ji-Hoon; Park, Seong-Kyu
- Abstract
Aim: To investigate the antioxidant effects of Morinda officinalis ( Morindae radix, MR) on H2O2-induced oxidative stress in cultured mouse TM3 Leydig cells. Methods: We carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells. Results: MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and C AT activity (74.6 units of CAT/mg protein, P < 0.001). Conclusion: These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress.
- Publication
Asian Journal of Andrology, 2008, Vol 10, Issue 4, p667
- ISSN
1008-682X
- Publication type
Article
- DOI
10.1111/j.1745-7262.2008.00414.x