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- Title
Training and acute exercise modulates mitochondrial dynamics in football players' blood mononuclear cells.
- Authors
Busquets-Cortés, Carla; Capó, Xavier; Martorell, Miquel; Tur, Josep; Sureda, Antoni; Pons, Antoni; Busquets-Cortés, Carla; Capó, Xavier; Tur, Josep A
- Abstract
<bold>Purpose: </bold>Regular physical activity induces oxidative stress but also causes adaptations in antioxidant defences including the nuclear factor κB (NF-κB) pathway, which activates target genes related to antioxidant defences such as uncoupling proteins (UCPs), and mitochondrial biogenesis mediated by peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). The aim of the study was to determine the effect of long-term training and acute exercise on oxidant/antioxidant status and the expression of mitochondrial biogenesis genes in peripheral blood mononuclear cells (PBMCs).<bold>Methods: </bold>Twelve professional football players performed an 8-week exercise programme comprising a daily 2-h football training session. Blood samples were taken before and after the training season.<bold>Results: </bold>The results reported a significant increase in antioxidant protein levels and in mitochondrial proteins in resting conditions after the 8-week training period. PGC1α, UCP-2 and mitofusin 2 protein levels also increased after acute exercise compared to pre-exercise levels. After the training, the expression of PGC1α, cytochrome c oxidase subunit IV and mitochondrial NADH dehydrogenase subunit 5 messenger RNA (mRNA) significantly augmented after the acute physical activity compared to pre-exercise levels; while no changes occurred in these mRNA in basal conditions. NF-κB activation and ROS production reported a significant increase after acute exercise.<bold>Conclusions: </bold>Training increases the levels of proteins related to mitochondrial biogenesis and improves the antioxidant capabilities of mitochondria in PBMCs among well-trained football players. Acute exercise may act as an inducer of mitochondrial biogenesis through NF-κB activation and PGC1α gene expression.
- Subjects
EXERCISE; SOCCER players; PHYSICAL activity; OXIDATIVE stress; PHYSIOLOGICAL effects of antioxidants; MONONUCLEAR leukocytes; PHYSIOLOGY; PROTEIN metabolism; ENZYME metabolism; ENZYMES; FOOTBALL; HYDROLASES; MITOCHONDRIA; MONOCYTES; PROTEINS; RESEARCH funding
- Publication
European Journal of Applied Physiology, 2017, Vol 117, Issue 10, p1977
- ISSN
1439-6319
- Publication type
journal article
- DOI
10.1007/s00421-017-3684-z