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- Title
Asthma induction in mice leads to appearance of alpha2-3- and alpha2-6-linked sialic acid residues in respiratory goblet-like cells.
- Authors
Kirkeby, Svend; Jensen, Niels-Erik Viby; Mandel, Ulla; Poulsen, Steen Seier
- Abstract
Allergic asthmatic inflammation in mice was induced by sensitization with ovalbumin and lipopolysaccharide from Escherichia coli and visualized in the airways of asthmatic mice by spatial and temporal changes of carbohydrates containing sialic acid residues. Immunohistochemistry was used to demonstrate binding of lectins and antibodies that detect alpha2-3- and alpha2-6-linked sialic acid residues. After sensitization and challenge, the histology of the lung changed markedly, and goblet-like cells appeared, most likely caused by Clara cell metaplasia. Normal Clara cells showed no reaction after incubation with the sialic acid detecting agents, while the goblet-like cells expressed both alpha2-3- and alpha2-6-linked sialic acid residues in the asthmatic animals. The lectins but not the antibodies reacted with intestinal goblet cells. Instead, an antibody recognizing a disialoganglioside, stained large mononuclear cells in the submucosa, indicating a difference in sialylation between goblet cells in the intestine and goblet-like cells developed from Clara cells.
- Subjects
CARBOXYLIC acids analysis; ANIMALS; ASTHMA; BIOLOGICAL models; CARBOXYLIC acids; EPITHELIAL cells; INTESTINES; LUNGS; MICE; ALBUMINS
- Publication
Virchows Archiv: European Journal of Pathology, 2008, Vol 453, Issue 3, p283
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-008-0645-2