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- Title
The developmental transcriptome of Drosophila melanogaster.
- Authors
Graveley, Brenton R.; Brooks, Angela N.; Carlson, Joseph W.; Duff, Michael O.; Landolin, Jane M.; Yang, Li; Artieri, Carlo G.; van Baren, Marijke J.; Boley, Nathan; Booth, Benjamin W.; Brown, James B.; Cherbas, Lucy; Davis, Carrie A.; Dobin, Alex; Renhua Li; Wei Lin; Malone, John H.; Mattiuzzo, Nicolas R.; Miller, David; Sturgill, David
- Abstract
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
- Subjects
DROSOPHILA melanogaster; TRANSGENIC organisms; GENOMES; RIBONUCLEASES; EXONS (Genetics)
- Publication
Nature, 2011, Vol 471, Issue 7339, p473
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature09715